@article{ZinsserKrysKapitzaBoehnkeetal.2022,
  author    = {Jillena Zinsser-Krys and Christopher Kapitza and Lena B{\"o}hnke and Piet van der Keylen and Winfried L. Neuhuber and J{\"u}rgen W{\"o}rl},
  title     = {Neurochemical classification of serotonin-immunoreactive neurons co-innervating motor endplates in the mouse esophagus},
  series   = {The Anatomical Record},
  volume    = {306},
  number    = {5},
  doi       = {10.1002/ar.25030},
  pages     = {960 -- 971},
  year      = {2022},
  abstract  = {Serotonin immunoreactivity was previously found in myenteric neurons co-innervating motor endplates in the mouse esophagus striated muscle and aninvolvement in motility control was suggested. However, it is not known ifother neuroactive substances are present in these neurons and to what extentthey co-localize. First, vasoactive intestinal peptide (VIP) was established as abona fide marker for putative inhibitory myenteric neurons by evaluating co-localization with neuronal nitric oxide synthase (nNOS) and neuropeptide Y(NPY). Then, co-localization of serotonin and VIP was tested in co-innervatingaxons on motor endplates, which were visualized withα-bungarotoxin (α-BT)by multilabel immunofluorescence. Myenteric ganglia were also surveyed forco-localization in neuronal perikarya and varicosities. nNOS, NPY, and VIPwere completely co-localized in enteric co-innervating nerve terminals onmotor endplates. After co-staining with VIP, we found (a) serotonin (5-HT)-positive nerve endings without VIP (44\% of 5-HT-positively innervated end-plates), (b) 5-HT- and VIP-positive endings without co-localization (35\%), and(c) 5-HT- and VIP-positive endings with co-localization (21\%). About one-fifthof nerve terminals on motor endplates containing 5-HT originate from putativeinhibitory peptidegic nitrergic neurons. However, the majority represents a different population presumably subserving different functions.},
  language  = {en}
}